Tag: Trizol
Sympathetic neuropeptide Y has a protective effect on the cardiovascular system
Cells were then labelled with EdU and cultured with or without 1M NPY or 2 M For mural cells, the medium is for 6 h and has low- Glucose DMEM with 2% FBS. The cells were imaged with a confocal microscope using Zen-Black software and having a 20/ 0.8 NA objective. The cells were collected 5 days later and either fixed with 4% PFA for imaging or lysed with Trizol for RNA extraction.
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