Tag: puromycin
UM171 glues to the asymmetric assembly to degrade the CoREST corepressors
Lentiviral particles carrying overexpression constructs were produced and used totransduce K562 KBTBD4-null CoREST–GFP cells. The particles were added with a final concentration of 40–0.006 M, and then incubated with DMSO for 72 h. The cells were selected with 2 g ml1 puromycin for three days, then sorted for single-cell clones on the BD FACSAria Cell Sorter (BD Biosciences).
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