Tag: importin
The HIV capsid is similar to FG-nucleoporins
An experimental method was developed using hexameric hexameric CAmer-mCherry and hexameric CASenmer-mCherry. The CAmer-mCherry was first incubated with importin-:importin-, TNPO1, TNPO3 (all final 2.5M). NaCl, 2 mM DTT, twice against 50 mM Tris pH 8, 1,000 mM NaCl, and twice against 50 mM Tris pH 8, 40 mM NaCl, and twice against 50 mM Tris pH 8, 40 mM NaCl, to remove nucleic acid contaminations.
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